Virology

Our recent publications regard two main areas:

Mapping and characterizing recessive resistance determinants in potyviruses with the aim of indentifying host factors involved in viral replication and spread

The mechanisms of gene silencing as an underlying mechanism of resistance against virus infection, and as a tool in functional genomics and plant transformation.

Publications

Braunstein, T.H., Moury, B., Johannessen, M. & Albrechtsen, M., 2002. Specific degradation of 3' regions of GUS mRNA in posttranscriptionally silenced tobacco lines may be related to 5'-3' spreading of silencing. RNA 8, 1034-1044.

Hjulsager, C.K., Lund, O.S. & Johansen, I.E., 2002. A New Pathotype of Pea seedborne mosaic virus Explained by Properties of the P3-6k1 and VPg Coding Regions. Molecular Plant-Microbe Interactions 15(2), 169-171.

Moury, B., Morel, C., Johansen, E. & Jacquemond, M., 2002. Evidence for diversifying selection in Potato virus Y and in the coat protein of other potyviruses. Journal of General Virology 83, 2563-2573.

Borgstrøm, B. & Johansen, I.E., 2001. Mutations in pea seed-borne mosaic virus genome-linked protein VPg alter pathotype specific virulence in Pisum sativum. Molecular Plant Microbe Interactions 14(6): 707-714

Johansen, I.E., Lund, O.S., Hjulsager, C.K. & Laursen, J., 2001. Recessive resistance in Pisum sativum and potyviral pathotype resolved in a gene-for-cistron correspondance between host and virus. Journal of Virology 75(14), 6609-6014. Order reprint

Libiakova, G., Jørgensen, B., Palmgren, G., Ulvskov, P. & Johansen, E., 2001. Efficacy of an intron containing kanamycin resistance gene as selectable marker in plant transformation. Plant Cell Reports 20, pp. 610-615.

Olsen, B. S., and Johansen I. E. 2001. Nucleotide Sequence and Infectious cDNA Clone of the L1 Isolate of Pea Seed-borne Mosaic Potyvirus. Archives of Virology 146: 15-25

Bak, S., Poulsen, G.B., Albrechtsen, M., 1998. Two cases of nonhost resistance against potyviruses operating at the single cell level. Journal of Phytopathology 146: 623-629.

Dalmay, T., Horsefield, R., Braunstein, T. H., and Baulcombe, D. C. 2001. SDE3 encodes an RNA helicase required for post-transcriptional gene silencing in Arabidopsis, EMBO J. 20: 2069-2078.

Jones, AL., Johansen, IE., Bean, SJ., Bach, I., Maule, AJ. (1998) Specificity of resistance to pea seed-borne mosaic potyvirus in transgenic peas expressing the viral replicase (Nlb) gene. Journal of General Virology, 79, 3129-3137.

Andersen K, Johansen IE. (1998) A single conserved amino acid in the coat protein gene of pea seedborne mosaic potyvirus modulates the ability of the virus to move systemically in Chenopodium quinoa. Virology, 241: 304-311.

Keller KE, Johansen IE, Martin, RR, Hampton, RO. (1998). Potyvirus genome-linked protein (VPg) determines pea seedborne mosaic virus pathotype-specific virulence in Pisum sativum. Molecular Plant Microbe Interactions, 11: 124-130.

Okamoto, D., Nielsen, S.V.S., Albrechtsen, M., Borkhardt, B. (1996) General resistance against potato virus Y introduced into a commercial potato cultivar by genetic transformation with PVY^N coat protein gene. Potato Research 39: 271-282

Johansen IE, Dougherty WG, Keller KE, Wang D, Hampton RO (1996) Multiple viral determinants affect seed transmission of pea seedborne mosaic virus in Pisum sativum Journal of General Virology 77: 3149-3154

Johansen IE. (1996) Intron insertion facilitates amplification of cloned viral cDNA in Escherichia coli while biological activity is re-established after transcription in vivo. Proc. Natl. Acad. Sci. USA. 93: 12400-12405

Johansen E, Edwards MC, Hampton RO (1994) Seed transmission of viruses: Current perspectives. Annu Rev Phytopathol 32: 363-386.